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Tamra Dye Newlin
Ponca City High School (1988 - 1992)
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Dove Medical Press - Fri, 30 Dec 2016
A purified single isomer 5 of amine-containing TAMRA dye. Often a reagent of choice for preparing TAMRA-labeled peptides.
Tetramethylrhodamine (TMR, TRITC) has been a widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Under the name TAMRA, the carboxylic acid of 5-TAMRA has also achieved prominence as a dye for oligonucleotide labeling and automated DNA sequencing applications.
Description 5 (6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. This bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm. For Research Use Only.
Fluorescent dye for DNA, peptides and proteins.
(5-Carboxytetramethylrhodamine) 5-TAMRA (λex/λem: 541/568nm in DMF or DMSO) is the purified single isomer of 5 (6)-TAMRA. It is one of the most popular …
TAMRA DyeTAMRA dyes 5(6)-Carboxyﬂuorescein (FAM) dyes are traditionally used for labeling of biomolecules. FAM is a green ﬂuorescent dye used to label peptides, proteins and nucleotides. The maximum excitation peak of FAM is closely matched to …
General description An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.
Background FAM, JOE, TAMRA and ROX, the "big four" dyes, have dominated DNA …
Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
www.biosearchtech.com CH-130423.02 0.0 0.1. 0.2. 0.3. 0.4. 0.5. 0.6. 0.7. 0.8. 0.9. 1.0. 400. 450. 500. 550. 600. 650. 700. 750. 800. Normalized Absorbance/Intensity ...
TAMRA is a well known xanthene dye of rhodamine series. This is a thiol reactive maleimide for the labeling of SH groups in proteins and peptides. Pure 6-isomer.
ChemPep: One stop for peptide synthesis. ChemPep provides custom services in peptide synthesis and peptide array. ChemPep is the leading supplier of amino acid building blocks, peptide coupling reagents, solid phase synthesis resins, fluorescent dyes, pharmaceutical peptides, catalog peptides and cosmetic peptides.
TAMRA dye is an effective quencher for fluorophores with emission maxima less than 560 nm. Dyes with longer wavelength emissions will not be effectively quenched by TAMRA. In addition, TAMRA has its own fluorescence which complicates data analysis due to crosstalk between the channels.
Rhodamine / ˈ r oʊ d əm iː n / is a family of related dyes, a subset of the triarylmethane dyes.They are derivatives of xanthene.Important members of the rhodamine family are Rhodamine 6G, Rhodamine 123, and Rhodamine B.They are mainly used to dye paper and inks, but they lack the lightfastness for fabric dying.
Mixed isomers of carboxytetramethylrhodamine (TAMRA) free acid. The dye can be coupled to primary or secondary amines via standard peptide chemistry. λ Ex /λ Em (MeOH) = 540/565 nm Dark red solid soluble in DMSO, DMF, MeOH, or pH >6.5 buffer
Quenchers may be fluorescent (TAMRA™) or nonfluorescent molecules (DABCYL, Black Hole Quencher ® (BHQ ®). For optimal performance, the quencher's absorbance spectrum should match the reporter’s emission spectrum as closely as possible. Recommended reporter/quencher combinations can be found in the Spectral Properties table. Table 2.
TAMRA (Carboxytetramethylrhodamine) is spectrally similar to iFluor 555, Alexa Fluor 555, Cy3 (Cyanine-3), Helix Fluor 575, and iFluor 560. TAMRA (Carboxytetramethylrhodamine) belongs to the following related categories: Classic Dyes, Peptide and Oligonucleotide Labeling, and Dyes by Functional Group.
Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright ...
ATTO TM 488 (NHS Ester) 502. 522. Alexa Fluor® 488 (NHS Ester) ATTO TM Dyes. Rhodamine Green TM -X (NHS Ester) 504.
It is used as a replacement for the TAMRA dye. Special Notes and Considerations: The CAL Fluor Red 590 modification is a suitable dye for Stellaris RNA FISH assays as it is both bright and stable. As with Quasar 570, this is a suitable option if you have a …
CID 2762603 (5-Carboxy methylrhodamine) Date s. Modify. 2021-05-29. Create. 2005-07-19. 5-carboxytetramethylrhodamine is a tetramethylrhodamine compound having a carboxy substituent at the 5-position It has a role as a fluorochrome.
This dye is supplied purified single isomer 5. 5-TAMRA has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.
TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore is being used for quite a long time for the preparation of dual labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM).
Quenchers: TAMRA™ Dye vs. MGB Fluorescent = Uses up one filter/dye spot Long (28-38bp) = less specificity Non-fluorescent Shorter (13-18bp) = higher specificity Sensitive to single base mismatch! 77 Real-time PCR Fluorescent Chemistries •Uses a dsDNA binding dye to …
A single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5' or 3' end of oligos.
5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm. - Mechanism of Action & Protocol.
General Strategy As you can see from above, there are up to 4 dyes that can be used to label the PCR products. The last dye (red/orange) is used for an internal lane standard.
Tamra Dye's Reputation Profile. Edit Profile. Review. Lock. Message. Court Records found View. Lawsuits, Liens or Bankruptcies found on Tamra's Background Report Criminal or Civil Court records found on Tamra's Family, Friends, Neighbors, …
5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. - Mechanism of Action & Protocol.
LightCycler ® 480 Detection Formats. Due to its highly flexible selection of excitation and emission wavelengths, the LightCycler ® 480 Real-Time PCR System is compatible with most currently available dyes and assay formats, yet is also adaptable to new, future applications.. The following assay formats can be used on the LightCycler ® 480 Real-Time PCR System:
• 384-Well Spectral Calibration Plate with TAMRA™ Dye (PN 4432296) • 384-Well Spectral Calibration Plate with NED™ Dye (PN 4432302) • 384-Well Region of Interest (ROI) and Background Plates (PN 4432320) • 384-Well Normalization Plates with FAM™/ROX™ and VIC®/ROX™ Dyes (PN 4432308)
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AxisPharm is a world leading provider for click chemistry. Folate-, Biotin-, and Peptide PEG linkers for target oriented bioconjugation research. Peptide Linkers PEG conjugates for enzyme specific cleavage. Working with the members of HT Laboratories/AxisPharm was an amazing experience.
This kit contains 16 TAMRA dye labeled single stranded DNA oligos for size standards. More . There are no specifications . Fragment Analysis Genotyping Sanger Sequencing No attachment. You May Also Like. add to cart quick view. EZ1 RNA Tissue Mini Kit (48) ...
quenched by TAMRA™ dye at the 3´ end or non-fluorescent quencher. MultiScribe Reverse Transcriptase MultiScribe™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (MuLV) Reverse Transcriptase (P/N N808-0018). MultiScribe Reverse Transcriptase is similar to MuLV Reverse Transcriptase, but differs in its recommended usage ...
Fluorescence quantum yield of the dyes correlates with the presence of dG nucleosides in the adjacent region of oligonucleotide sequence. Several energy transfer primers containing on their 5'-termini (6-FAM)dT(n)(6-TAMRA) dye system (n = 0, 2, 4, 6, 8, 10, 12, 14) were prepared, and their spectral properties were studied.
Description. These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC™, ROX™, SYBR™ Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification.
Simple to use protein labeling kits for covalent labeling of antibodies or other proteins with fluorescent dyes. AFDye 488 Antibody Labeling Kit. Labeling Target: Lys (primary amine) Abs/Em: 494/517 nm. PRICING. Catalog#. Unit Size. Price (USD) Availability.
TAMRA PEG4-Alkyne [ Catalog No. KWG1025 ] - Click chemistry dyes. 1 mg $100.00 5 mg $250.00 25 mg $500.00
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•TAMRA™ dye-quenched probe Small 125 – Inventoried (20 ) Medium 500 Large 2500 ‡See “Step 3: Order a candidate endogenous control assay” on page 36. Product Type 20-µL rxns. Part number Availability
Three-component Förster resonance energy transfer (FRET) has been used to obtain efficient FRET between the cationic conjugated polymer (CCP) as donor and 5-carboxy tetramethylrhodamine (TAMRA) dye as acceptor, by using an intermediate donor, fluorescein. In spite of the fact that there is enough ov …
Glen Research CE (β-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate ...
6-TAMRA Cadaverine CAS# 709025-58-1 formule: C30H34N4O4 purity: ≥95%. 3000+ high purity PEG Linkers in stock. Support custom synthesis.Fast Shipping.
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over TAMRA™ dye–quenched probes. The use of MGB probes increases the probability of designing an assay in traditionally difficult sequence regions (i.e., AT-rich sequences). Additionally, we have found that the relatively short MGB probes increase the probability that we can design a …